پایش تنوع ژنهای کد کننده مقاومت آنتی بیوتیکی در فاضلاب های شهری

Background and objective: Wastewater treatment plants are important hotspots for the spread of antibiotic resistance genes to the environment.  The aim of this study identification of relevant antibiotic resistance genes in raw and final effluent of municipal wastewater treatment and also determination of effects of different wastewater treatment process on removal/reduction of these pollutants. Materials and Method: Samples were taken on basis standard condition and transferred with temperature preserved. Six genes that coding resistance to six current antibiotics were selected.  PCR examination was used for identification (presence/absence) of antibiotic resistance genes. Results: The overall, identified Antibiotic resistance genes in raw and final effluents are 85.1 and 59.4 percent, respectively. Comparision of results shows antibiotic resistance genes              in  domestic raw wastewater are sul1 (100%)  and ctx-m-32(55.5%) the highest and lowest and in final effluent sul1(88.89%) and aac3-I(33.35) the highest and lowest, respectively. Conclusion: This study was shown efficiency of activated sludge process is better than stabilization ponds. And also diversity of these agents in wastewater treatment is high and wastewater treatment effects is very different.